Rapid, single-step most-probable-number method for enumerating fecal coliforms in effluents from sewage treatment plants
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چکیده
منابع مشابه
Rapid, single-step most-probable-number method for enumerating fecal coliforms in effluents from sewage treatment plants.
A single-step most-probable-number method for enumerating fecal coliforms in sewage treatment plant effluents is described. The method requires the use of only one lactose-based medium and a single incubation temperature of 44.5 degrees C, and it can be completed in 18 h or less, as compared with up to 72 h for the standard most-probable-number method. The appearance of growth is the sole crite...
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Fecal coliforms growing in a selective lactose-based broth medium at 44.5 degrees C generate a change in the electrical impedance of the culture relative to a sterile control when populations reach 10(6) to 10(7) per ml. The ratio of these changes was measured automatically, and the data were processed by computer. A linear relation was found between the log10 of the number of fecal coliforms i...
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The standard one-step M-FC broth-membrane-filter procedure for recovery of fecal coliforms from chlorinated sewage effluents is much less effective than the multiple-tube (most-probable-number) technique. A two-step membrane-filter method, using a pre-enrichment technique with phenol red lactose broth and incubation at 35 degrees C for 4 h, followether 18+/-2 h, enhanced fecal coliform recovery...
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Total coliform (TC), fecal coliform (FC), and fecal streptococcus (FS) recoveries in chlorinated secondary sewage effluents were investigated by the membrane filter (MF) and multiple-tube (most probable number, MPN) methods. The LES two-step MF method was found to be comparable to the MPN procedure for determining TC. The TC detection was 1.5 times greater by the LES two-step technique than tha...
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We used six rhizobium-legume systems to test the reliability of the most-probable-number (MPN) technique for enumerating rhizobia introduced into 14 sites representing four soil orders. The range-of-transition values (the number of dilution steps between the first not-entirely-positive and the last not-entirely-negative growth units) were compared for each species and for each soil. The probabi...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1979
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.37.3.527-530.1979